Drug response vs protein abundance (scatter plot)

This plot shows the relationship between (y-axis) and (x-axis). Each dot represents a multiple myeloma patient sample (bone marrow biopsy), colored by their respective PhenoGroup as follows:
●=PG1 , ●=PG 2 , ●=PG3 , or  ●=PG not measured.

is measured by Pharmacoscopy for the myeloma cells of each patient sample. The drug response is expressed as the Pharmacoscopy score, i.e. 1 - (% myeloma cells in drug condition / % myeloma cells in control condition). Positive values indicate the relative fraction reduction of myeloma cells. 0 indicates no change in the myeloma fraction upon drug perturabtion. And negative values indicate toxicity, i.e. healthy cells died faster in response to the drugs compared to the myeloma cells in the patient sample.

is measured by Dia-SWATH based proteomics of purified CD138+ cells from the same patient bone marrow biopsies. The values represent z-score normalized log10-transformed protein intensities, i.e. standard deviations (sigma) from the mean (0), after regressing out the contribution of small plasma-marker positive cells. E.g. a +3 means the sample expressed this protein at 3 sigma above the average across the cohort.

This graph shows the biological network of top-correlating proteins with all tested drug responses. Rectangles are proteins (names indicated), edges indicate previously reported string-db connections. Proteins are colored according to their Spearman correlation coefficient with .

Top correlated proteins with drug response (bar plot)

This plot shows the Spearman correlation coefficients (x-axis) of the top-4 and bottom-4 proteins (y-axis) most strongly correlated with across all measured MM patient samples.

is measured by Pharmacoscopy for the myeloma cells of each patient sample. The drug response is expressed as the Pharmacoscopy score, i.e. 1 - (% myeloma cells in drug condition / % myeloma cells in control condition). Positive values indicate the relative fraction reduction of myeloma cells. 0 indicates no change in the myeloma fraction upon drug perturabtion. And negative values indicate toxicity, i.e. healthy cells died faster in response to the drugs compared to the myeloma cells in the patient sample.

Protein expression is measured by Dia-SWATH based proteomics of purified CD138+ cells from the same patient bone marrow biopsies. The values represent z-score normalized log10-transformed protein intensities, i.e. standard deviations (sigma) from the mean (0), after regressing out the contribution of small plasma-marker positive cells. E.g. a +3 means the sample expressed this protein at 3 sigma above the average across the cohort.